With the increasing advancement of stem cell-based therapeutic methods and various applied technologies such as low-level laser therapy, as a safe technique with extraordinary therapeutic potential, the use of this method has opened a new window in medicine. This study also investigates the effects of this radiation on markers of survival and migration of umbilical cord endothelial cells. Umbilical cord endothelial cells were obtained from the Biopajouh Afza Laboratory and were cultured in vitro under appropriate conditions and culture medium. Cell viability was measured 24 and 48 hours after laser irradiation using the MTT method. To examine cell migration, a wounding method using a sampler and photography of the cell migration trajectory were used. Image analysis was performed using GraphPad Prism 4 software (GraphPad Software, Inc., San Diego, CA, USA). Two-way analysis of variance (ANOVA) was used to analyze the data, and Tukey's test was used to compare the means. The values were calculated as mean ± standard deviation and the difference was considered significant at a probability level of less than 5%. Comparison of the percentage of viability of different samples between the two time points of 24 and 48 hours showed that there was no statistically significant difference in the comparative times (P<0.05) and at both times the percentage of cell viability in the group treated with a wavelength of 660 nm was significantly different from the control group (P<0.0001). The results of photographing the migration of endothelial cells at times 0, 24 and 48 hours showed that the highest cell migration occurred after 48 hours and in the sample treated with a laser with a wavelength of 660 nm. The results of the present study indicate that stimulation of cells with low-power laser increases the growth, viability and migration of vascular endothelial cells.

Laser Radiation Cell